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Huge Microbiology Blood Training Complete Collection

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Training Skills Direct




Training Skills Direct Microbiology and Blood Collection



Bacteriology Blood Electrolytes and Intravenous Infusions Clinical Chemistry Epidemiology and Microbiology Hematology Immunohematology and Blood Banking Immunology Laboratory Mathematics Microbiology for the Veterinary Specialist Mycology Parasitology Urinalysis



Microbiology and Blood Collection Microbiology and Blood Collection Bundle CD-ROM brought to you by Training Skills Direct. 12 Training Course Collection 2547 Pages 16 Training Manuals 74 Complete Lessons Complete Installation Package Either run this training course bundle from the CD-ROM or install directly onto your computer with full access from your Windows Start menu. The training courses are provided in PDF format and they are fully indexed for easy navigation. Box image used for illustration purposes only. You will receive a CD-ROM in a protective sleeve and mailer. This listing is for a CD-ROM. You will not receive a hard copy book. Available for Windows only. Not compatible with Mac OS X.

Bacteriology Training Course Training Manual - Bacteriology (266 Pages) Lesson 1 - Introduction Section I. Host-parasite Relationships Causation of Microbial Disease Terminology The Infectious Process Section II. Aspects of Pathogenicity Capsulation Bacterial Toxins Extracellular Enzymes Section III. Host Resistance Nonspecific Factors Specific Factors-Immunological Response Section IV. The Bacterial Cell Autotrophic Bacteria Heterotrophic Bacteria Morphology Structure of Bacteria Identification of Bacteria Pathogenic Bacteria Section V. Collecting and Processing of Bacteriological Specimens Basic Principles Collecting and Processing of Blood Cultures. Collecting and Processing of Specimens from Therespiratory Tract Collecting and Processing Urine Specimens Collecting and Processing of Fecal Specimens Collecting and Processing Body Fluids Collecting and Processing Exudate Specimens Processing of Specimens for Shipment Exercises Solutions Lesson 2 - Microscopic Examination and Cultivation of Bacteria Section I. Microscopic Examination of Bacteria Preparation of Smears for Staining Acid-Fast Stain (Ziehl-Neelsen) Acid-Fast Stain (Kinyoun Modified) Capsule Outline (India Ink) Capsule Stain (Hiss Method) Flagella Stain (Leifson Method Modified) Gram Stain (Hucker's Modification) Methylene Blue Stain Spore Stain (Wirtz-Conklin) Staining of Blood Smears Preparation of Wet Unstained Mounts for Microscopicexamination Section II. Cultivation of Bacteria (Media) Composition of Media Hydrogen Ion Concentration (pH) Section III. Environmental Factors Temperature Oxygen Requirements Carbon Dioxide Requirements Section IV. Anaerobic Methods Basic Principles Collection and Transport of Clinical Materials Examination of Smears Inoculation of Primary Isolation Media Incubation and Subculture Procedure After Incubation Section V. Antibacterial Agents, Sterilization, Andaseptic Technique Antibacterial Agents General Comments on Sterilization Media Preparation Heat Filtration Radiation General Comments on Aseptic Technique Working Area Disposal of Contaminated Materials Personal Cleanliness Section VI. Isolation of Bacteria Inoculating Loops and Needles Streak Plate Pour Plate Method Test Tube Cultures Exercises Solutions Lesson 3 - Common Tests and Gram-Positive Cocci Section I. Common Tests Bile Solubility Test Carbohydrate Fermentation Test Catalase Test Citrate Test Coagulase Test Ferric Chloride Test Gelatin Liquefaction Test Hydrogen Sulfide Test (Lead Acetate Paper Test) Indole Test Methyl Red Test Nitrate Reduction Test Oxidase Test Urease Test Voges-Proskauer (VP) Test Section II. Gram-Positive Cocci: Staphylococci Pathogenicity of Staphylococci Cultural Characteristics of Staphylococci Laboratory Indentification of Staphylococci Confirmatory Procedures Section III. Gram-Positive Cocci: Streptococci Introduction to the Streptococci Hemolytic Patterns Pathogenicity of the Streptococci Cultural Characteristics of the Streptococci General Identification Streptococci Beta Hemolytic Streptococci Differentiation of Other Streptococci Section IV. Gram-positive Cocci: Pneumococci Morphology of Pneumococci Growth Requirements of Pneumococci Colony Characteristics of Pneumococci Pathogenicity of Pneumococci Laboratory Identification of Pneumococci Confirmatory Studies Exercises Solutions Lesson 4 - Gram-Negative Cocci and Gram-Positive Bacilli Section I. Introduction General Comments About the Neisseriae Common Characteristics of the Neisseriae Section II. Neisseria Gonorrhoeae Pathegenicity Criteria for Women Criteria for Men Special Situations Obtaining Specimens for Culture - Women Obtaining Specimens for Culture - Men Conditions for Inoculation of Modified Thayer-Martin Medium Modified Thayer-Martin Medium in Plates Co2-Generating Tablets Modified Thayer-Martin Medium Bottles Incubation Presumptive Identification of Neisseria Gonorrhoeae Confirmatory Identification of Neisseria Gonorrhoeae Arbohydrate Utilization Tests for Neisseria Gonorrhoeae Confirmation of Neisseria Gonorrhoeae Section III. Neisseria Meningitidis Pathogenicity Similarity to Gonococci Source Identification Section IV. Gram-Positive Bacilli: Corynebacteriaand Related Species General Comments About the Gram-Positive Bacilli General Comments About the Genus Corynebacterium Cultivation of Corynebacteria Pathogenicity of Corynebacteria Laboratory Identification of Corynebacteria Listeria Monocytogenes Erysipelothrix Rhusiopathiae Section V. Gram-Positive Bacilli: Bacillus Species Bacillus Spore Formation Bacillus Cultures Pathogenicity of Bacillus Laboratory Identification of Baci-species Section VI. Gram-Positive Bacilli: Clostridia Growth Requirement of Clostridia Appearance of Clostridia Pathogenicity of Clostridia Laboratory Identification of Clostridium Species Section VII. Gram-Positive Bacilli: Mycobacteria Pathogenicity General Procedures Appearance of Mycobacteira Tration for Cultivation Cultivation of Mycobacteria Virulent Strains of Mycobacteria Saprophytic Mycobacteria Confirmatory Procedues for Mycobacteria Catalase Test (for Acid-Fast Bacilli) Neutral Red Test (for Acid-Fast Bacilli) Niacin Test (for Acid-Fast Bacilli) Exercises Solutions Lesson 5 - Enterobacteriaceae Section I. Introduction Normal Flora and General Identification of Species Morphology and Culture Section II. Pathogenicity of Enterobacteriaceae Pathogenicity of the Genus Escherichia Pathogenicity of the Genus Klebsiella Pathogenicity of the Genus Enterobacter Pathogenicity of the Genus Proteus Pathogenicity of the Genus Salmonella Pathogenicity of the Genus Shigella Section III. Enteric Media Categories of Enteric Media Lactose Fermentation Differential Media Selective Media Enriched Media Tubed Differential Media Isolation of Enteric Pathogens from Fecal Specimens Section IV. Preliminary Screening of Cultures Forenterobacteriaceae Colony Characteristics Use of Less Inhibitory Media IMViC Tests Section V. Identification of Enteric Organisms Lysine Decarboxylase Test (Falkow Method) Decarboxylase Tests (Moeller Method) Phenylalanine Deaminase Test Malonate Test Pectolysis Test (Martin and Ewing) Motility Test The Proteus and Providencia Genera The Enterobacter and Klebsiella Genera The Genus Salmonella The Genus Shigella The Genus Escherichia Morphology Cultural Characteristics Laboratory Identification Pathogenicity Exercises Solutions Lesson 6 - Other Pathogenic Gram-Negative Bacilli and Antibiotic Sensitivity Tests Section I. Pseudomonas Pseudomonas Aeruginosa Pseudomonas Pseudomallei Section II. Brucella General Types Culture Pathogenicity Laboratory Identification Section III. Haemophilus Morphology Cultural Characteristics Pathogenicity and Identification Section IV. Bordetella Identification Section V. Francisella Tularensis Morphology Cultural Characteristics Laboratory Identification Pathogenicity Section VI. Miscellaneous Organisms Bacteroides Fragilis Streptobacillus Moniliformis Section VII. Antibiotic Sensitivity Tests Antibiotic Resistance Preventing Emergence of Resistant Strains of Bacteria Laboratory Antibiotics Methods for Determining Sensitivityof an Organism to Antibiotics Kirby-Bauer Method for Determining Bacterial Sensitivity Exercises Solutions Blood Electrolytes and Intravenous Infusions Training Course Training Manual - Blood, Electrolytes and Intravenous Infusions (79 Pages) Lesson 1 - Human Blood Section I. Blood Parts Properties and Functions of the Blood Blood Cells Section II. Function of the Blood in Trauma-Hemostasis Temporary Plug Muscle Constriction Clot Formation Section III. Blood Types or Groups Major Groups A-B-O Group Rh Factor Other Blood Groups Exercises Solutions Lesson 2 - Fluids and Electrolytes Section I. Body Fluids Normal Fluid Content in the Body Processes for Maintaining Water Balance Categories of Fluids Section II. Electrolytes in Body Fluids Electrolytes Acid-base Balance Nature of Solutions Diffusion and Osmosis Crystalloid and Colloid Solutions Normal Fluid Loss Abnormal Fluid Loss Replacing Abnormal Fluid Loss Enzymes Hormones Exercises Solutions Lesson 3 - Intravenous Preparations and Methods for Administration Section I. Infusions Intravenous Preparations Categories of Intravenous Fluids and Their Uses Hydrating Solutions Electrolyte Replacement Solutions Nutrient Solutions (Hyperalimentation Products) Requirements for Intravenous Solutions/Intravenous Admixtures Precautions for Using Fluid Therapy Site for Venipuncture Principles of Intravenous Therapy Complications Calculating the Intravenous Drip Rate Explanation of Drip Rate Formula Example of a Drip Rate Calculation Section II. Transfusions Products for Transfusion Site for Venipuncture When a Blood Product Is Used Rate of Transfusion Adverse Blood Product Reactions General Principles of Transfusion Blood Tests Section III. Infusion Procedures Equipment Required for the Infusion Procedure for Venipuncture Discontinue an Intravenous Infusion Special Considerations for Transfusions Section IV. Venous Cutdown Reasons for Using the Venous Cutdown Venous Cutdown Procedure Exercises Solutions Appendix - Glossary of Terms Clinical Chemistry Training Course Training Manual 1 - Clinical Chemistry 1 (205 Pages) Lesson 1 - Laboratory Safety Section I. Safety Principles Safety Program Material Safety Data Sheets (MSDS) Work Area Section II. Volatile and Hazardous Materials Volatile Flammables Hazardous Materials Exercises Solutions Lesson 2 - Collection, Preservation and Shipment of Specimens Section I. Collection and Preservation of Specimens Collection and Preservation of Blood Specimens Collection and Preservation of Serum Collection and Preservation of Cerebrospinal Fluid Collection and Preservation of Serous Fluid Collection and Preservation of Synovial Fluid Collection and Preservation of Amniotic Fluid Collection of Urine Preservation of Urine Collection of Fecal Specimens Section II. Criteria for Collection and Acceptance of Specimens Collection Variables Criteria for an Unacceptable Sample Section III. Shipment of Specimens Shipment of Specimens Chain of Custody Exercises Solutions Lesson 3 - Measurement of Weights and Volumes Section I. Measurement of Weights Balances Principles of Weighing Types of Balances Mechanical Balances Electronic Balances Proper Use and Care of the Balance Performance Section II. Measurement of Volume Volumetric Glassware Volumetric Flasks Manual Pipettes Automatic and Other Kinds of Pipettes and Dispensers Burets Factors in the Selection and Use of Pipettes Cleaning of Glassware Exercises Solutions Lesson 4 - Introduction to Quality Control Section I. Quality Control System Quality Control System Common Errors Section II. Quality Control in Clinical Chemistry Quality Control in Clinical Chemistry Mean (Arithmetic) Average Standard Deviation Percent Coefficient of Variation Establishing a Quality Control Program Constructing and Interpreting a Quality Control Chart (Levey-Jennings) Westgard Multi-Rule Chart Quality Control Review Exercises Solutions Lesson 5 - Introduction to Organic Chemistry Section I. Introduction to Basic Concepts Significance of Organic Chemistry Carbon and Basic Organic Structures Section II. Classes of Organic Compounds Hydrocarbons Alcohols Aldehydes Ketones Organic Acids Esters Ethers Amines Amides Thiols (Mercaptans) Exercises Solutions Training Manual 2 - Clinical Chemistry 2 (97 Pages) Lesson 1 - Carbohydrates Section I. Introduction to Carbohydrates Chemical Composition of Carbohydrates Nomenclature of Carbohydrates The Classification of Carbohydrates The Metabolism of Carbohydrates Section II. Glucose Determination Body Sources of Glucose The Clinical Significance of Abnormal Blood Sugar Levels The Glucose Oxidase Method of Glucose Determination The Colormetric Method - Ortho-Toluidine The Ultraviolet (UV) Method The Glucose Tolerance Test (GTT) Section III. Hormonal Control of Blood Glucose Levels Insulin Glucagon Glucocorticoids Epinephrine Anterior Pituitary Hormones Exercises Solutions Lesson 2 - Lipids Section I. Lipids Introduction to Lipids Definition Functions of Lipids Classification of Lipids Section II. Fats Introduction to Fatty Acids Section III. Triglycerides Introduction to Triglycerides The Structure of Triglycerides Properties of Triglycerides Metabolism of Triglycerides Analysis of Triglyceride Section IV. Cholesterol Introduction to Cholesterol Structure of Cholesterol Properties of Cholesterol Metabolism of Cholesterol Clinical Significance of Cholesterol The Theoretical Considerations of Cholesterol Analysis The Analysis of Cholesterol by the Cholesterol Oxidase Method Alternative Determination of Total Cholesterol (Fe+++, H2SO4 Method) The High-Density Lipoprotein (HDL) Cholesterol Determination (Precipitation Method) Section V. Lipoproteins Clinical Significance of Inherited Defects in Lipoprotein Patterns The Analysis of Lipoproteins by Electrophoresis The Normal Range Lipoproteins Section VI. Total Lipids Introduction to Total Lipids Exercises Solutions Lesson 3 - Proteins Section I. Introduction to Proteins Body Functions Performed by Proteins Chemical Composition of Proteins Chemical Structure of Proteins Importance of Proteins to the Laboratory Technician Total Protein, Albumin and Globulin Section II. Total Protein Determination The Determination of Total Protein in Serum The Clinical Significance of Abnormal Levels of Serum Protein Other Methods of Total Protein Determination Section III. Albumin Determination Albumin Determination Clinical Significance of Abnormal Levels of Albumin in the Serum Globulin Albumin/Globulin (A/G) Ratio Section IV. Cerebrospinal Fluid (CSF) Protein Determination Cerebrospinal Fluid Protein Determination Clinical Significance of Abnormal Levels of Protein in the CSF Preference for the Tca Method Section V. Urine Protein The Semiquantitative Screening Test for Urine Protein Additional Semiquantitative Test for Urine Protein Quantitative Test for Urine Protein The Clinical Significance of Abnormal Levels of Protein in the Urine Bence-Jones Proteins Section VI. Routine Electrophoresis General Principles of Routine Electrophoresis The Electrophoretic System Normal Values for Electrophoresis The Clinical Significance of the Electrophoresis Procedure Section VII. Immunofixation Electrophoresis Immunoglobulins Immunofixation Electrophoresis Immunoelectrophoresis Section VIII. Fibrinogen Importance of Fibrinogen The Source and Function of Fibrinogen The Fibrinogen Molecule The Clinical Importance of Fibrinogen Levels in the Plasma The Value of a Fibrinogen Screening Test The Fibrinogen Screening Test Other Tests for Fibrinogen Exercises Solutions Epidemiology and Microbiology Training Course Training Manual - Epidemiology and Microbiology (88 Pages) Lesson 1 - Introduction Disease Transmission and Epidemiology Responsibilities for Health Definitions Communicable Diseases The Chain of Disease Transmission Disease Agents Sources of Disease (Reservoirs) Means of Disease Transmission (Mode of Transmission) Susceptible Person (Host) Multiple Causation Communicable Disease Control Epidemiology Uses of Epidemiology Exercises Solutions Lesson 2 - Public Health Microbiology Section I. Introduction Types of Cells Types of Cell Reproduction Classification of Organisms Observation and Measurement Section II. Bacteria Morphology of Bacteria Structure of Bacteria Identification of Bacteria Pathogenic Bacteria Rickettsiae, Chlamydiae and Mycoplasmp Section III. Viruses Morphology and Physiology of Viruses Bacteriophages Pathogenic Viruses Section IV. Fungi Morphology of Fungi Physiology of Fungi Classification of Fungi Pathogenic Fungi Section V. Protozoa Classification of Protozoa Pathogenic Protozoa Classification of Helminths Cestodes (Tapeworms) Trematodes (Flukes) Nematodes (Roundworms) Exercises Solutions Lesson 3 - Practical Application of Microbiology Section I. Microbiology of Water and Sewage Sewage Microbiology Section II. Disinfection and Sterilization Physical Agents Chemical Agents Section III. Packing Microbiological Specimens for Shipment Importation and Interstate Shipment of Human Pathogens and Related Materials Exercises Solutions Hematology Training Course Training Manual 1 - Hematology 1 (231 Pages) Lesson 1 - Blood The Composition of Blood Formation of Blood Cells Normal Cell Maturation Abnormal Cell Maturation Functions of Blood Cells Exercises Solutions Lesson 2 - Material Employed in Hematology Laboratory Reagents Laboratory Glassware Exercises Solutions Lesson 3 - Collection of Blood and Preparation of Blood Smears Collection of Blood Specimens Preparation and Staining of Blood Smears Exercises Solutions Lesson 4 - Morphology of Blood Cells General Information Erythrocytes Leukocytes Thrombocytes The Leukemias Exercises Solutions Lesson 5 - Manual Cell Counts Manual Counts, Blood Specimens Manual Counts, Other Body Fluids Exercises Solutions Lesson 6 - Hematocrit, Erythrocyte Sedimentation Rate and Hemoglobin Hematocrit Eryghrocyte Sedimentation Rate Hemoglobin Exercises Solutions Appendix - Glossary of Terms Training Manual 2 - Hematology 2 (191 Pages) Lesson 1 - Differential Leukocyte Count and Other Procedures Differential Leukocyte Count Erythrocyte Indices and Fragility Tests Demonstration of L.E. Cells Special Stains Exercises Solutions Lesson 2 - Blood Coagulation Introduction Coagulation System The Coagulation Mechanism Coagulation Studies Exercises Solutions Lesson 3 - Anemia Diagnosing Anemia Laboratory Assessment of Anemia Anemia as Categorized by Morphology Exercises Solutions Lesson 4 - Leukemias Morphologic Disorders of the Leukocyte Classification of Leukemias Diagnostic Special Stains Exercises Solutions Appendix - Glossary of Terms Immunohematology and Blood Banking Training Course Training Manual 1 - Immunohematology and Blood Banking 1 (135 Pages) Lesson 1 - Blood Collection, Processing, Storage and Shipment Section I. Blood Collection and Processing Blood Donors Blood Donor Registration Donor Selection Medical History of Potential Donors Permanent Deferment of Donors Twelve Month Deferment of Donors (In Case Hepatitis Develops) Special Donor Categories The Collection of Blood Materials and Instruments Used in Blood Collection Blood Containers The Identification of Materials Preparation of the Venipuncture Site Phlebotomy and Collection of Samples for Processing and Start Care of the Donor After Phlebotomy Donor Reactions Processing Requirements for Donor Blood Section II. Blood Storage and Shipment Background Information Anticoagulant Preservatives Refrigeration of Blood Transportation of Blood Transportation of Frozen Components Donor Blood Inspection Bacteriologic Procedures Requirements for Reissuance of Blood Transportation of Infected Reagents Exercises Solutions Lesson 2 - Immunogenetics Section I. Blood Group Immunogenetics Genetic Terminology Gene Action Dominant and Recessive Traits Modes of Inheritance Gene Interaction Immunogenetic Systems Section II. The ABO System Genetic and Biochemical Considerations Cell and Serum Activity General Principles for All Serologic Testing Routine Testing for ABO Less Common Phenotypes Special Procedures-test for Hemolysins Discrepancies Between Cell and Serum Results Section III. The Rh System Rh-Positive and Rh-Negative Other Major Antigens Inheritance and Nomenclature Rh Variant (Du) Other Rh Antigens The LW Problem Rhnull Syndrome Rh Antibodies Prevention of Rho(D) Immunization Rh-Typing Tests for Antigens Other Than Rho(D) General Considerations in Rh-Testing Section IV. Other Blood Group Systems Lewis System I Blood Group System Kell Blood Group System P Blood Group System Duffy Blood Group System Kidd Blood Group System MNSs Blood Group System Lutheran Blood Group System Exercises Solutions Training Manual 2 - Immunohematology and Blood Banking 2 (195 Pages) Lesson 1 - Immunohematology Section I. The Immune Response and the Interaction of Antigens, Antibodies and Complement in Immunohematology The Immume Response Antigens Antibody Synthesis Primary and Secondary Immune Responses Immunization to Blood-Group Antigens Antibody Structure, Function and Properties Antigen-Antibody Reactions in Blood Group Serology Agglutination Hemolysis Complement The Classic Complement Pathway The Alternative Complement Pathway Stability of Complement with Particular Reference to Thedetection of Blood-group Antibodies Section II. The Antiglobulin Test Principles of the Antiglobulin Test Direct Antiglobulin Test Indirect Antiglobulin Test Role of Complement in the Antiglobulin Reaction Mechanisms for Sensitization of Red Blood Cells by Complement Standardization of Antiglobulin Serums Polyspecific Antiglobulin Reagents Monospecific Antiglobulin Serums Factors Affecting the Antiglobulin Test Sources of Error Section III. Red Blood Cell Antibodies Alloantibodies Procedures Reading and Interpretation of Reactions Reagent Red Blood Cells Serum Controls Antibody Detection Antibody Identification Use of Red Blood Cell Panels Antigenic Frequency Interpretation of Common Panel Results Antibodies Not Easily Identified Useful Additional Notes Cold Antibodies Titration Absorption Elution Section IV. Determination of Compatibility Determination of Compatibility Collection of Blood from Recipient Pretransfusion Testing of the Recipient Selection of Blood Massive Transfusion Techniques for Crossmatching Incompatible Crossmatch Labeling and Release of Crossmatched Blood Retention and Storage of Blood Samples Release of Blood in Emergency Situations Crossmatch in Presence of Prolonged Clotting Time Compatibility Testing After Infusion of Synthetic Plasma Expanders Exercises Solutions Lesson 2 - Immune Hemolytic Anemia and Hemolytic Diseases of the Newborn Section I. Investigation of a Positive Direct Antiglobulin Test and Immune Hemolytic Anemia Immune Hemolytic Anemia General Serologic Investigations Specificity of Autoantibody Cold Agglutinin Titer. Thermal Amplitude and Confirmation of Anti-l(i) Specificity ABO and Rh Grouping Autoimmune Hemolytic Anemia Associated with Warm Antibodies Autoimmune Hemolytic Anemia Associated with Cold Antibodies Drug-Induced "In Vivo" Red Blood Cell Sensitization Laboratory Investigation Op Drug-related Problems Detection of Antibodies to Penicillin and Cephalothin Section II. Hemolytic Disease of the Newborn Classification Mechanisms of Maternal Immunization Transfer of Antibody to the Fetus Clinical and Laboratory Features of Hemolytic Disease Prenatal Studies Laboratory Investigation of Hemolytic Disease of Newborns During the Neonatal Period Treatment of Hemolytic Disease of the Newborn Rho(D) Immune Globulin (Human)-RhIG Massive Fetomaternal Hemorrhage Maternal Alloimmunization to Platelets, Neutrophils and Protein All O Types Exercises Solutions Lesson 3 - Blood Transfusion Section I. Blood Transfusion Practice Transfusion of Blood Products Containing Red Blood Cells Transfusion of Platelets Blood Products Used to Replace Plasma Coagulation Factors Plasma Substitutes Special Situations Involving Transfusion Administration of Blood Products Section II. Adverse Effects of Blood Transfusion Immediate Effects Delayed Effects Records of Transfusion Complications Section III. Prevention of Hepatitis Background Selection of Donors Testing for Hepatitis B Surface Antigen and Hepatitis C in Blood Recipient Follow-up for Post-transfusion Hepatitis Hepatitis-implicated Donors and Those Found to Have Hepatitis B Surface Antigen or Hepatitis C Virus Use of Gamma Globulin with Transfusions Laboratory Safety Exercises Solutions Immunology Training Course Training Manual - Immunology (130 Pages) Lesson 1 - Immunity Section I. Introduction to Immunity Types of Immunity Section II. Immune Response Specific Immune Response Antigen Elimination Induction of the Immune Response Theories of Immunoglobulin Formation Nonspecific Responses Represent the Body's Initial Exercises Solutions Lesson 2 - Overview of the Immune System Section I. Antigens Immunogenicity Section II. Antibodies Structure Characteristics of Immunoglobulin Classes Section III. Complement Roles of Complement The Classical Pathway The Alternative Pathway The Membrane Attack Complex (MAC) Control Mechanisms Biological Effects of Complement Activation Exercises Solutions Lesson 3 - Cells of the Immune System Section I. Introduction Lymphoid Tissues Lymphocytes Development of B Cells and T Cells Macrophages Section II. B Cells and Humoral Immunity Antigen Processing and Presentation Antigen Triggering of Specific T Cells B Cell Activation Antibody and Memory B Cell Production Section III. T Cells and Cellular Immunity Antigen Stimulated T Cells Biologic Expression Exercises Solutions Lesson 4 - HLA Complex Section I. Immunogenetics Genetics of Immune Regulation Section II. HLA Complex Nomenclature Classifications Exercises Solutions Lesson 5 - Immunological Techniques Section I. Introduction Context Application Section II. Precipitation Reactions Precipitation Reactions Immunodiffusion Section III. Serum Protein Electrophoresis (SPE) Principle Interpretation Section IV. Immunoelectrophoresis (IEP) Principle Monoclonal Gammopathies Section V. Enzyme Immunoassay Techniques Types of Enzyme Immunoassays Conjugate Enzymes Substrates Clinical Applications Exercises Solutions Lesson 6 - Antinuclear Antibodies and Testing Section I. Immunofluorescent Microscopy Immunofluorescent Methods The Fluorescent Microscope Section II. Antinuclear Antibodies Related Diseases Antinuclear Antibodies Section III. Fluorescent Antinuclear Antibody Test (FANA) Reporting Results Nuclear Patterns Limitations Exercises Solutions Lesson 7 - Viral Immunity Section I. Virology Structure of a Virus Classification of Viruses Section II. Human Immunodeficiency Virus History of Human Immunodeficiency Virus Classification Structure Human Immunodeficiency Virus Replication and Transmission Human Immunodeficiency Virus-cell Interactions Damage to the Immune System Laboratory Detection Section III. Viral Hepatitis Symptoms of Hepatitis Causative Agents Hepatitis Immunological Markers Section IV. Hepatitis A Virus Virus Structure Mode of Transmission Incubation Period and Infectivity Human Immunodeficiency Virus Immunological Assays Section V. Hepatitis B Virus Virus Structure Mode of Transmission Incubation Period and Infectivity Hepatitis B Virus Immunological Assays Types of Hepatitis B Virus Infections Section VI. Hepatitis D Virus (Hepatitis B Virus, Hepatitis D Virus) Virus Structure Mode of Transmission Incubation Period and Infectivity Hepatitis D Virus Immunological Assays Types of Hepatitis D Virus Infections Section VII. Hepatitis Non-A, Non-B Virus Structure Mode of Transmission Non-B Immunological Assays Exercises Solutions Laboratory Mathematics Training Course Training Manual - Laboratory Mathematics (181 Pages) Lesson 1 - General Mathematics Review Section I. Properties of Real Numbers Some Fundamental Properties of Real Numbers Definitions of Subtraction and Division Properties of Zero Properties of Negative Numbers Properties of Fractions Order of Operations Exercises Section II. Properties of Equality Properties of Equality Rules of Equality Solving First Degree Equations Problem-Solving Techniques Exercises Section III. Rounding Off Numbers Rules Exercises Section IV. Scientific Notation Exponents Rules of Exponentiation Expressing Numbers in Terms of Scientific Notation Changing the Sign of the Exponent Multiplication of Numbers Expressed in Scientific Notation Division of Numbers Expressed in Scientific Notation Exercises Section V. Significant Figures Determining the Number of Significant Figures in a Number Implied Limits Laboratory Application Exercises Section VI. Logarithms Examples Parts of a Logarithm Determination of the Characteristic Determination of the Mantissa Determination of the Logarithm of a Number Logarithms of Numbers Expressed as Exponentials Determination of Antilogarithms Use of Logarithms in Multiplication Use of Logarithms in Division Use of Logarithms to Find Roots of Numbers Use of Logarithms to Find the Product of Numbers with Exponents Exercises Section VII. Solutions to Exercises Solutions to Exercises Lesson 2 - Introduction to Solution Mathematics Section I. Grades of Laboratory Chemicals Standards Reagent Grade or Analytical Reagent Grade Organic Grade Chemically Pure Grade Pharmacopeia and National Formulary Grades Purified, Practical, or Pure Grades Technical or Commercial Grades Section II. Grades of Laboratory Water Type I Water Type II Water Type III Water Minimum Specifications for the Three Types of Water Requirements for Standards and Reagents Carbon-Dioxide-Free Water Frequency of Water Testing Section III. Parts of a Solution Solvent Solute Examples Section IV. Types of Solutions Colloidal Solution Emulsions Saturated Solutions Unsaturated Solutions Section V. Percent Concentration Weight/Volume Percent Solutions Solving Weight/Volume Problems Hydrates Volume/Volume Percent Solution Problems Weight/Weight Percent Solutions Percent Composition Problems Advantages and Disadvantages of Percent Solutions Exercises Solutions Lesson 3 - Molar Solutions Section I. Mole Concept Calculating Gram Molecular Weight Moles and Gram Molecular Weight Section II. Molarity and Molar Solutions Solving Molarity Problems Section III. Millimoles Per Liter Discussion Solving Millimole per Liter Problems Section IV. Variations of Molarity Problems Problems Involving Hydrates Problems Involving Only Part of the Molecule Advantages and Disadvantages of Molar Solutions Exercises Solutions Lesson 4 - Equivalent Solutions Section I. Equivalent Weight Determining the Gram Equivalent Weight Exceptions in Calculating Gram Equivalent Weight Equivalents Section II. Normal Solutions Solving Normality Problems Section III. Milliequivalent per Liter (mEq/L) Solutions Discussion Solving Milliequivalent per Liter Problems Section IV. Variations of Normality Problems Problems Involving Hydrates Problems That Consider Only Part of the Molecule Exercises Solutions Lesson 5 - Conversion of Concentration Units Conversion from mol/L to g/dL and from g/dL to mol/L Conversion from Eq/L to g/dL and from g/dL to Eq/L Converting from mol/L to Eq/L and from Eq/L to mol/L Exercises Solutions Lesson 6 - Dilutions Simple Serial Dilutions Preparation of a Working Solution from a Stock Solution Preparation of Standards by Mixing Exercises Solutions Lesson 7 - Titration Indicators and General Rules of Use Performing an Acid-base Titration Solving Titration Problems Calculating the Reacting Strength of a Solution Exercises Solutions Lesson 8 - Concentrated Acids and Bases Example Specific Gravity and Density Preparing Solutions from Concentrated Liquids Problem Solving Determining the Concentration of Concentrated Liquids Exercises Solutions Lesson 9 - pH and Buffers Section I. Introduction Arrhenius Concept Bronsted-Lowry Theory Weak Acids/Bases Versus Strong Acids/Bases Section II. Dynamic Equilibrium Law of Mass Action Example Section III. Dissociation of Water Discussion Ion Product of Water Section IV. pH and pOH Concept of pH Application of Concept of pH pOH pH Scale pOH Scale Relationship Between pH and pOH Section V. Hydrolysis of Salts Hydrolysis of the Salt of a Weak Acid and a Strong Base Hydrolysis of the Salt of a Strong Acid and a Weak Base Hydrolysis of the Salt of a Weak Acid and a Weak Base Section VI. Buffers Mechanism of Action The Henderson-Hasselbalch Equation Solving Buffer Problems Exercises Solutions Appendix A - Review of Dimensional Analysis Appendix B - Table of Four-Place Logarithms Appendix C - Table of Common Elements and Radicals Appendix D - Metric Prefixes Microbiology for the Veterinary Specialist Training Course Training Manual - Microbiology for the Veterinary Specialist (113 Pages) Lesson 1 - Introduction to Microbiology Section I. Introduction Significance Section II. Microbiological Relationships Causation of Microbial Disease Terminology The Infectious Process Section III. Aspects of Pathogenicity Capsulation Bacterial Toxins Extracellular Enzymes Section IV. Host Resistance Nonspecific Factors Specific Factors-immunological Response Section V. The Bacterial Cell Autotrophic Bacteria Heterotrophic Bacteria Morphology Structure of Bacteria Identification of Bacteria Pathogenic Bacteria Section VI. Collecting and Processing of Bacteriological Specimens Basic Principles Section VII. Microscopic Examination of Bacteria General Comments Gram Stain Section VIII. Cultivation of Bacteria Composition of Media Hydrogen Ion Concentration (pH) Section IX. Environmental Factors Temperature Oxygen Requirements Carbon Dioxide Requirements Section X. Anaerobic Methods Basic Principles Section XI. Antibacterial Agents, Sterilization and Aseptic Technique Antibacterial Agents General Comments on Sterilization Media Preparation Heat Filtration Radiation General Comments on Aseptic Technique Working Area Disposal of Contaminated Materials Personal Cleanliness Section XII. Isolation of Bacteria Inoculating Loops and Needles Streak Plate Pour Plate Method Test Tube Cultures Exercises Solutions Lesson 2 - Microorganisms Causing Foodborne Illness and Other Diseases of Publc Health Significance Section I. Introduction to Foodborne Illness Causes Chemicals Toxicants Microbiological Agents or Their Toxins Section II. Staphylococci General Comments About Gram-positive Cocci Pathogenicity of Staphylococci Cultural Characteristics of Staphylococci Section III. Streptococci Introduction to the Streptococci Hemolytic Patterns Pathogenicity of the Streptococci Section IV. Corynebacteria and Related Species General Comments About the Gram-positive Bacilli General Comments About the Genus Corynebacterium Pathogenicity of the Corynebacteria Listeria Monocytogenes Erysipelothrix Rhusiopathiae Section V. Bacillus Species General Comments About the Genus Bacillus Bacillus Spore Formation Bacillus Cultures Pathogenicity of Bacillus Section VI. Clostridia General Comments About the Genus Clostridium Growth Requirements of Clostridia Appearance of Clostridia Pathogenicity of Clostridia Section VII. Mycobacteria General Comments About the Genus Mycobacterium Pathogenicity Section VIII. Enterobacteriaceae Normal Flora and General Identification of Specie Morphology and Culture Pathogenicity of the Genus Escherichia Pathogenicity of the Genus Klebsiella Pathogenicity of the Genus Enterobacter Pathogenicity of the Genus Proteus Pathogenicity of the Genus Salmonella Pathogenicity of the Genus Shigella Section IX. Yersinia Pestis Morphology Pathogenicity Section X. Pseudomonas Pathogenicity of Pseudomonas Aeruginosa Pseudomonas Pseudomallei Section XI. Brucella General Types Culture Pathogenicity Section XII. Bordetella Culture Section XIII. Francisella Tularensis Morphology Cultural Characteristics Pathogenicity Section XIV. Miscellaneous Organisms Streptobacillus Moniliformis Vibrio Parahaemolyticus Exercises Solutions Lesson 3 - Food Spoilage Due to Microorganisms Section I. Introduction Causes of Spoilage Section II. Bacterial Spoilage of Various Food Products Bacterial Spoilage of Vegetables Bacterial Spoilage of Fruits Bacterial Spoilage of Fresh and Cured Meats, Poultry and Waterfoods Bacterial Spoilage of Eggs Bacterial Spoilage of Dairy Products Bacterial Spoilage of Canned Foods Section III. Molds Introduction to Molds and Yeasts Introduction to Molds Molds in Food Section IV. Yeasts Introduction Yeasts in Food Exercises Solutions Mycology Training Course Training Manual - Mycology (177 Pages) Lesson 1 - Introduction to Medical Mycology History Classification of Fungi Cultivation Macroscopic Appearance of Fungi Microscopic Structures Mycoses Exercises Solutions Lesson 2 - Collection and Processing Procedures for Mycological Studies Abscess Specimens Biopsy Material Blood Cultures Bone Marrow General Spinal Fluid Hair, Skin and Nails (Dermatophytes) Draining Fistula, Sinus Tract Joint Fluid Nasopharyngeal Pleural Fluid Sputum, Bronchial Washings Thrush Lesions of Oral Cavity Urine Specimen Exercises Solutions Lesson 3 - Saprophytic Fungi Section I. Introduction Reasons for Studying Potential Clinical Importance Opportunistic Infections Section II. Zygomycetes Mucor Species Rhizopus Species Syncephalastruh Species Absidia Species Section III. Deuteromycetes Introduction to Deuteromycetes (Fungi Imperfecti) Aspergillosis Alternaria Cladosporium Drechslera Curvularia Penicillium Paecilohyces Nigrospora Scopulariopsis Trichoderma Fusarium Sepedoniuh Exercises Solutions Lesson 4 - Yeasts of Medical Importance Candidiasis Candida Albicans Candida Tropicalis Differentiation of Candida Species Geotrichum Candiduh Cryptococcus Rhodotorula Torulopsis Saccharomyces Exercises Solutions Lesson 5 - Cutaneous Fungi Section I. Superficial Fungi Malassezia Furfur Exophiala Herneckii Piedraia Hortae Trichosporon Bbigblli Section II. Dermatophytes Microsporum SPP Epidermophyton Floccosuh Trichophyton Diseases Caused by Dermatophytes Exercises Solutions Lesson 6 - Subcutaneous Fungi Mycetoma Chromomycosis Rhinosporidiosis Sporothrichosis Exercises Solutions Lesson 7 - Systemic Mycoses Histoplasmosis Blastomycosis Paracoccidioidomycosis Coccidioidomycosis Exercises Solutions Lesson 8 - Mycological Procedures Section I. Specimen Processing Potassium Hydroxide with Glycerol India Ink Reagents and Test Lactophenol Cotton Blue Stain Slide Culture Section II. Organism Identification Germ Tube Test Sterile Rice Grain Test Urease Test In Vitro Hair Test Periodic Acid-Schiff Stain Saline Wet Mount Cut-Streak Method for Morphology of Yeasts Exercises Solutions Lesson 9 - Media and Reagents Section I. Media Isolation Media Sabouraud's Dextrose Agar Sabouraud Dextrose Agar with Antibiotics (Mycoseltm) Brain Heart Infusion Agar Potato Dextrose Agar Cornmeal Agar Cream of Rice Agar with Tween 80 Germ Tube Media Rice Grains Section II. Reagents Lactophenol Cotton Blue Mounting Media Potassium Hydroxide Solutions Periodic Acid-Schiff Stain Reagents Exercises Solutions Lesson 10 - Maintaining Stock Cultures Maintenance of 'Working' Stock Cultures Placing Stock Cultures in Sterile Distilled Water Placing Stock Cultures Under Oil Recovering Organisms from Water Stock Recovering Organisms from Oil Shock Exercises Solutions Lesson 11 - Commercial Kits API* 20C Identification System Minitektm Yeast Carbon Assimilation Test Uni-Yeast-Tektm Yeast Identification System API* Yeast-Identtm System Exercises Solutions Appendix - Glossary of Terms Parasitology Training Course Training Manual 1 - Parasitology 1 (122 Pages) Lesson 1 - Introduction to Parasitology Section I. Overview of Parasitology History of Parasitology Types of Organism Relationships Terms Important in Parasitology Parasitic Attributes That Influence Disease Host Attributes That Influence Resistance to Pathogenic Effects of Parasitism Epidemiology Preventive Measures The Evolution of Parasites Section II. Safety and Quality Control in the Parasitology Laboratory Individual Health Hazards Disposal of Contaminated Materials Handling of Hazardous Reagents Equipment in the Parasitology Laboratory Stains and Reagents Continuing Education Section III. Taxonomy of Parasites Infecting Humans Divisions of Living Organisms Phylum Protozoo Phylum Platyhelminthes Phylum Aschelminthes Phylum Acanthocephahelminthes Section IV. Microscopy Equipment Care of the Microscope Use of the Microscope Operation of the Binocular Microscope Parfocality Kohler Illumination Light Intensity The Use of the Oil Immersion Objective Scanning Techniques The Stereoscope Exercises Solutions Lesson 2 - Collecting, Preserving and Processing Clinical Specimens Section I. Overview Types of Specimens Preservations of Specimens Processing Serological Diagnosis Section II. Blood Specimens Collection of Specimens The Venipuncture The Capillary Puncture Preservation of Blood Anticoagulants Fixation and Storage Processing the Blood Smear Section III. Gastrointestinal Tract Specimens Collection of Gastrointestinal Tract Specimens Macroscopic Examination Occult Blood in Feces Preservation Processing of Gastrointestinal Specimens Stain Reactions Cultivation of Parasites Exercises Solutions Appendix - Glossary Training Manual 2 - Parasitology 2 (253 Pages) Lesson 1 - Collecting, Preserving and Processing Clinical Specimens Section I. Overview of Protozoo History Structure Parasitic Protozoo Section II. Class Rhizopoda Locomotion and Digestion Respiration and Reproduction Amoebic States Section III. Class Zoomastigophora Lumen Flagellates Blood and Tissue Flagellates Exercises Solutions Lesson 2 - Phylum Protozoo: Ciliata, Piroplasmasida and Sporozoa Section I. Class Ciliata Respiration and Reproduction Parasitic Member of Class Ciliata Section II. Class Piroplasmasida Class Function Parasitic Member of Class Piroplasmasida Section III. Class Sporozoa Family Eimeriidae Family Endodycoccidoridae Family Plasmodiidae Organisms of Class Sporozoa Exercises Solutions Lesson 3 - Phylum Platyhelminthes Section I. Overview of Platyhelminthes Structure Reproduction Classes Section II. Class Trematoda Subclasses Common Parasites of Class Trematoda Section III. Class Cestoda Orders Common Parasites of Class Cestoda Exercises Solutions Lesson 4 - Phylum Aschelminthes: Phylum Acanthocephahelminthes: Arthropods and Vectors Section I. Phylum Aschelminthes Nematode Groups Blood and Tissue Nematodes Section II. Phylum Acanthocephelminthes Macracanthorhynchus Hirudinaceus Section III. Arthropod and Vectors Morphological Characteristics Exercises Solutions Appendix A - Clinical Manifestations and Treatment Appendix B - References Appendix C - Medical Parasitology Urinalysis Training Course Training Manual - Urinalysis (84 Pages) Lesson 1 - The Collection and Preservation of Specimens; Macroscopic and Physical Examination of Urine Section I. Collection and Preservation of Specimens Importance of Urinalysis Random Specimen First Morning Void Two-Hour Postprandial Twenty-Four Hour Specimen Special Methods of Urine Collection Preservation Section II. Macroscopic and Physical Examination of Urine Volume Color General Appearance of the Urine Sample Specific Gravity pH Odor Foam Exercises Solutions Lesson 2 - Chemical Measures Specific Gravity pH Glucose Ketones Proteins Proteins in Urine Blood Bilirubin Urobilinogen Bacteriuria: Nitrite Bacteriuria: Leukocyte Esterase Exercises Solutions Lesson 3 - The Microscopic Examination of Urinary Sediment Section I. Preparation and Illumination Preparation of Urine Sediment for Examination (Unstained) Basic Technique Illumination of Microscopic Field Common Sources of Error Section II. Microscopic Examination of Organized Sediment Red Blood Cells (Erythrocytes) White Blood Cells (Leukocytes) Epithelial Cells Casts - General Comments Noncellular Casts Cellular Casts Pseudocasts Section III. Microscopic Examination of Unorganized Sediment Normal Crystals Found in Acid Urine Abnormal Crystals Found in Acid Urine Crystals Found in Alkaline Urine Extraneous Structures Introduction Formula Discussion Procedure White Blood Cells Epithelial Cells Casts Miscellaneous Structures Exercises Solutions





PDF Format Training Course This training course bundle is provided in PDF format. The course reads like a textbook on your computer screen and you can use the bookmarks to navigate through the chapters or lessons. Adobe Reader 9 or above is required to view each course and has been included on the CD-ROM. The courses are included digitally on the CD-ROM so you will not receive a hard copy book or textbook. We offer full customer support so please contact us if you need any help. Complete Installation Package This is a complete software installation program and provided in CD-ROM format. You can install each training course directly onto your computer with full access from your Windows Start menu. Alternatively you can simply run each training course from the CD-ROM without the need for an installation. The CD-ROM You will receive a professionally produced and printed CD-ROM in a protective sleeve and mailer. The CD-ROM includes a start menu to assist you with the easy installation of your new software. Simply insert the disc into your computer and the menu will begin instantly. The box image used is for illustration purposes only. Please note that there is no physical book or textbook included. The training courses are included digitally on the CD-ROM in PDF format. All the courses in this bundle will be included on the same CD-ROM. System Requirements This CD-ROM is compatible with the following operating systems: Windows 2000 Windows 2003 Server Windows XP Windows Vista Windows 7 Adobe Reader 9 or above is required to launch the training course and has been included on the disc. *This training course is not available for Mac OS X.



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Copyright This training course is copyrighted; any copying, duplicating, reproducing, licensing or sub licensing is strictly prohibited. The training course is security protected. The persons and models used are for illustrative purposes only. Copyright © Training Skills Direct. All rights reserved.

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