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- DescriptionThe field of oligonucleotide therapeutics research is ripe with the prospect of new discoveries. In Therapeutic Oligonucleotides: Methods and Protocols, a selection of established and emerging methods for the application of oligonucleotides as therapeutics are presented, all providing the tools needed to inspire great changes in the field. Divided into twenty-one chapters, this detailed volume meticulously describes vital protocols for optimizing and improving cell uptake, such as photochemical internalization, modified cell penetrating peptides, antibody conjugates, and naparticles. Other chapters address quantitation of RNA therapeutics in cells, assaying gene kckdown, selecting the best target site and synthesis of various modified oligonucleotides. Written in the successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tes on troubleshooting and avoiding kwn pitfalls. Authoritative and easily accessible, Therapeutic Oligonucleotides: Methods and Protocols serves as a timely resource for both professionals and vices pursuing research in this exciting and pioneering field.
- PublisherHumana Press Inc.
- Date of Publication15/07/2011
- SubjectClinical Medicine: Professional
- Series TitleMethods in Molecular Biology
- Series Part/Volume Number764
- Place of PublicationTotowa, NJ
- Country of PublicationUnited States
- ImprintHumana Press Inc.
- Content Note70 black & white illustrations, 7 black & white tables, biography
- Weight1830 g
- Width178 mm
- Height254 mm
- Spine20 mm
- Edited byJohn Goodchild
- Format DetailsLaminated cover
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