A dramatic increase in the number of practical techniques available to the experimental neuroanatomist have led to major advances in the kwledge and understanding of the nervous system. This book is a laboratory manual for experimental neuroscientists, with an emphasis on ultrastructural analysis. It contains detailed protocols for the preparation of specimens, perfusion-fixation, and the preparation of sections for light and electron microscopy. It covers neuroanatomical techniques including procedures for tract-tracing and chemical characterization of neurones and their processes. These procedures, either alone or in combination, are being used to elucidate complex neuronal interactions. This text should be useful to neuroanatomists and other neuroscientists who wish to apply these experimental techniques.