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- DescriptionThe second edition of this volume provides vel protocols for the study of newly discovered function and modifications of components of the Hh pathways. The first few chapters discuss methods for stable delivery of soluble Hh proteins in cell cultures that can be adapted for in vivo studies, and mathematical models for studying Hh proteins gradient formation in tissues. The next few chapters explore studies in cell culture using fibroblasts that respond readily to Hh proteins. The book goes on to present protocols for evaluation of transcriptional activity of the Gli transcription factors in cell cultures and determine the expression level of endogeus Gli proteins. The final chapters talk about metabolic effects of ncanical Hh signaling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding kwn pitfalls. Comprehensive and authoritative, Hedgehog Signaling Protocols, Second Edition presents researchers with a comprehensive understanding of the Hh pathway and its function in development and diseases.
- PublisherHumana Press Inc.
- Date of Publication21/07/2015
- SubjectLife Sciences: General
- Series TitleMethods in Molecular Biology
- Series Part/Volume Number1322
- Place of PublicationTotowa, NJ
- Country of PublicationUnited States
- ImprintHumana Press Inc.
- Content Note14 black & white illustrations, 29 colour illustrations, 8 black & white tables, biography
- Weight618 g
- Width178 mm
- Height254 mm
- Spine18 mm
- Edited byNatalia Riobo
- Edition Statement2nd Revised edition
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