In the past ten years there has been ermous progress in the development of eukaryotic viral vectors. In general, these vectors have been developed for one of three reasons: to achieve high levels of expression of a particular gene product (poxvirus, baculovirus, and adevirus), to clone eukaryotic genes in combination with functional assays (Epstein-Barr virus), of for use as delivery vehicles for the stable introduction of foreign genes into mammalian cells (retroviruses, Epstein-Barr virus, and ade-associated virus). Each vector has its strengths and weaknesses that are rooted in the sometimes bewildering stra- tegies that the parent viruses use for propagation. No one of these vectors is appropriate for all of the problems that a mole- cular biology laboratory is likely to encounter, and few of us are kwledgeable in the molecular virology of all of these viruses. This volume represents an attempt by the authors to assem- ble a review of these vectors in one place and in a form useful to laboratories that do t necessarily have experience with eukaryotic viruses. Clearly, any virus can be modified to serve as a vector for some purposes, and it was t possible to include a description of all of these. In addition, one eukaryotic vector, SV40 (the first one developed), has been reviewed so widely that we saw reason to include it here.
Springer-Verlag Berlin and Heidelberg GmbH & Co. KG
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Clinical Medicine: Professional
Current Topics in Microbiology and Immunology
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Springer-Verlag Berlin and Heidelberg GmbH & Co. K
5 Tables, black and white; 5 Illustrations, black and white; IX, 176 p. 5 illus.