How to use the Ovulation test strip

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One Step LH Urine Ovulation Test is fast and easy-to-use. It is a qualitative test that can predict when there is a LH (luteinizing hormone) surge, and in turn, when you are likely to ovulate and conceive.


First, you must determine the length of your menstrual cycle. This is the number of days from the first day of your menstrual bleeding to the day before your next bleeding begins again. Please refer to the chart below to determine when you should start testing. If your cycle is shorter than 21 days or longer than 38 days, consult your doctor. If you do not know your cycle length, you may begin the test 11 days after your first period since the average cycle length is 28 days. Perform 1 test each day over a 5 day period or until the LH surge has been detected.


1. Do not use first morning urine samples as LH is synthesized in your body early in the morning. It will not show up in your urine until later in the day.

2. The best time to collect your urine is between 10am - 8pm.

3. Collect urine at about the same time each day.

4. Reduce liquid intake about 2 hours before collecting your urine as a diluted urine sample can prevent the test from detecting LH surge.

Please refer to the table below for information on when to start using ovulation tests:

Your cycle length            Start to test on
21 days                            Day 6
22 days                            Day 6
23 days                            Day 7
24 days                            Day 7
25 days                            Day 8
26 days                            Day 9
27 days                            Day 10
28 days                            Day 11
29 days                            Day 12
30 days                            Day 13
31 days                            Day 14
32 days                            Day 15
33 days                            Day 16
34 days                            Day 17
35 days                            Day 18
36 days                            Day 19
37 days                            Day 20
38 days                            Day 21



Bring the test pouch to room temperature (18-30°C). To begin testing, open the sealed pouch by tearing along the notch. Remove the test from the pouch.


Immerse the strip into the urine with the arrow end pointing towards the urine. Do not immerse past the "Mark" line. Take the strip out after 20seconds and lay the strip flat on a clean, dry, non-absorbent surface (e.g., mouth of the urine container).


Wait for colored bands to appear. Depending on the concentration of LH in the test specimen, positive results may be observed in as short as 40 seconds. However, to confirm negative results, the complete reaction time (10 minutes) is required. Do not read results after 30 minutes.


     __                               __
     |X|                              |X|
     |X|                              |X|
     |X|                              |X|
     |X|                              |X|
     |X|                              |X|
     |X|                              |X|
     |_ |                             |_|    < control band
     |   |                             |  |   
     |_ |                             |  |    < test band
     |   |                             |  |
     |   |                             |  |
     |   |                             |  |
     |M|                             |M|
     |A|                             |A|
     |X|                              |X|
     |...|                             |..|
     |   |                             |  |
     |   |                             |  |
     |_ |                             |_|

Positive                      Negative

No LH Surge: Only one color band appears on the control region or the test band is present but lighter in color intensity than the control band. There is no LH surge even if two lines present - as long as test line is fainter than the control line the result is negative.
LH Surge: If two color bands are visible and the test band is equal to or darker than the control band, one will probably ovulate in the next 24-48 hours. If one wants to be pregnant, the best time to have intercourse is after 24 but before 48 hours.    

Invalid - Uncertain Results: No visible bands in the control and test regions. Make sure to follow the above specified instructions for optimum results.


1. For single in vitro diagnostic use only - use test only once for urine screening.
2. Do not use test kit beyond expiration date.
3. Keep away from moisture, direct sunlight, and children.
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