This volume aims to describe clearly and in detail the key practical skills involved in cartilage tissue engineering. Methods are outlined for isolation and expansion of chondrocytes and stem cells; differentiation; synthesis and application of three-dimensional scaffolds; design and operation of bioreactors; in vivo testing of engineered constructs; and molecular and functional analysis of cartilage cells and tissues. Frequently used technologies are covered, as well as more recent advances in inspirational areas such as 'smart' biomaterial development, novel bioreactor design, -omics analysis, and genetic manipulation of matrix synthesis. The key procedures discussed either underpin the progress already achieved in cartilage tissue engineering or are indicative of the direction of future research in the area. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cartilage Tissue Engineering: Methods and Protocols is a useful resource that informs the scientific community about the experimental work covering a broad range of objectives for cartilage synthesis and regeneration.
Product Identifiers
Publisher
Humana Press Inc.
ISBN-13
9781493929375
eBay Product ID (ePID)
214358969
Product Key Features
Subject Area
Bioengineering
Author
Pauline M. Doran
Publication Name
Cartilage Tissue Engineering: Methods and Protocols
Format
Hardcover
Language
English
Subject
Biology
Publication Year
2015
Type
Textbook
Number of Pages
290 Pages
Dimensions
Item Height
254mm
Item Width
178mm
Volume
1340
Item Weight
7856g
Additional Product Features
Series Title
Methods in Molecular Biology
Country/Region of Manufacture
United States
Editor
Pauline M. Doran
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