Next generation sequencing techniques allow for more detailed analysis of exons and introns in multiple genes at the same time. This will reveal many mutations that potentially lead to exon skipping. To functionally test these a lot can be achieved with a limited set of protocols, while for the intentional induction of exon skipping different tools and target genes are involved and the translational path from in vitro splicing to in vivo tests in animal models requiring a more extensive set of protocols. Exon Skipping: Methods and Protocols provides scientist with a comprehensive guide to many of the methods and techniques used for exon skipping, such as methods on how to discriminate real polymorphisms from mutations that affect splicing. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical Exon Skipping: Methods and Protocols seeks to aid scientists in the continuing study of exon skipping.
Product Identifiers
Publisher
Humana Press Inc.
ISBN-13
9781617797668
eBay Product ID (ePID)
113690514
Product Key Features
Author
Annemieke Aartsma-Rus
Publication Name
Exon Skipping: Methods and Protocols
Format
Hardcover
Language
English
Subject
Biology
Publication Year
2012
Type
Textbook
Number of Pages
440 Pages
Dimensions
Item Height
254mm
Item Width
178mm
Volume
867
Item Weight
1041g
Additional Product Features
Series Title
Methods in Molecular Biology
Country/Region of Manufacture
United States
Editor
Annemieke Aartsma-Rus
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