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Immunohistochemistry Is Highly Sensitive and Specific for Detection of Braf V600e Mutation in Pleomorphic Xanthoastrocytoma by Applied Research Press (Paperback / softback, 2015)

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About this product

Product Information

High frequencies of the BRAF V600E mutation have been reported in pleomorphic xanthoastrocytoma (PXA). Recently, a BRAF V600E mutation-specific antibody has been developed and validated. We evaluated the immuhistochemical (IHC) detection of BRAF V600E mutation in PXA by comparing to gold standard molecular analysis and investigating the interobserver variability of the IHC scoring. We performed BRAF V600E IHC in 46 cases, of which 37 (80%) cases had sufficient tumor tissue for molecular analysis. IHC detection was performed using moclonal mouse antibody VE1 (Spring Bioscience). IHC slides were scored independently by four reviewers blind to molecular data, including a primary (gold standard) and three additional reviewers. BRAF V600E mutation status was assessed by allele-specific polymerase chain reaction (PCR) with fragment analysis.We conclude that detection of BRAF V600E mutation by immuhistochemistry is highly sensitive and specific. BRAF V600E IHC interpretation is usually straightforward, but awareness of possible nspecific staining is necessary and training is recommended. It is a practical rapid method that may avoid the need of labor-intensive molecular testing and may be most valuable in small biopsies unsuitable for molecular analysis. Proceeds from the sale of this book go to the support of an elderly disabled person.

Product Identifiers

Publisher
Createspace Independent Publishing Platform
ISBN-10
1516825462
ISBN-13
9781516825462
eBay Product ID (ePID)
223085962

Product Key Features

Author
Applied Research Press
Format
Paperback / Softback, Trade Paperback (US) ,Unsewn / Adhesive Bound
Language
English
Subject
Life Sciences: General
Type
Textbook

Additional Product Features

Content Note
Colour Illustrations
Date of Publication
09/08/2015
Country of Publication
United States

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