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Manipulating the Mouse Embryo: A Laboratory Manual, Fourth Edition by Richard Behringer (Paperback, 2014)

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This 4th edition of the Mouse Manual -Manipulating the Mouse Embryo-appears 28 years after the first edition and once again is the definitive reference source on mouse development, transgenesis techniques, and molecular biology. While many of the techniques described in earlier editions of this manual have been relegated to core facilities, advances in DNA sequencing techniques and genome analysis have opened new avenues of research and developments. New approaches, such as the derivation of induced pluripotent stem cells and new targeted gene manipulation techniques that enable direct injection of RNA/DNA constructs into zygotes to achieve gene targeting, require new explanations and protocols. Authors Richard Behringer, Marina Gertsenstein, Kristina Nagy, and Andras Nagy-pre-emininent leaders in their fields-have taken these developments into account in updating and rewriting this fourth edition to include new information and protocols on: - generation of induced pluripotent stem cells - RNA microinjections - lentiviral microinjections and infection - assisted reproduction techniques for sperm and embryo cryopreservation - isolation, generation, and transplantation of spermatogonial stem cell lines - in utero electroporation of gene constructs into postimplantation embryos - vibratome sectioning of live and fixed tissues for imaging thick tissue sections - whole-mount fluorescent staining methods for three-dimensional visualization In addition, the wealth of essential information on mouse laboratory strains, mouse housing and breeding, surgical procedures, assisted reproduction, handling of embryos, and micromanipulation setups has been entirely updated. The first edition of Manipulating the Mouse Embryo appeared in 1986 as an outgrowth of Cold Spring Harbor Laboratory courses on the molecular embryology of the mouse held in the early 1980s, and authors of the first two editions included Brigid Hogan, Rosa Beddington, Frank Costantini, and Liz Lacy. The field's technological sophistication has grown exponentially since then, but the manual remains the essential practical and theoretical guide for all students, lab technicians, and investigators who work with mice. I have the third edition. Why should I buy the fourth edition? The new technologies regarding induced pluripotent stem cells and RNA injection and CRISPR technology are changing the field and opening up new avenues of investigation. The protocols for these as well as other up-to-the minute techniques in the fourth edition are by the leading proponents in the field and offer clear step-by-step guidance with copious illustrations. To whom is this edition addressed-the neophyte or the experienced investigator? Both groups can benefit from this manual. For the neophyte, the summary of mouse development and the how-to's of setting up a working laboratory are an invaluable introduction to the field. For the experienced investigator, the detailed protocols of new techniques in the field, with extensive troubleshooting and over-the-shoulder advice, allow easy integration of these techniques into a laboratory's research objectives. Are the protocols in this book better than those available for download on the Web? Many of the protocols available on the Web are perfectly good, but there are many that are not. Reagents are expensive and deadlines are tight. CSHL Press manuals provide protocols that have been formulated and tested in the labs of leading investigators in the field. They are reliable and they work. And they include the context and troubleshooting information that many online protocols do not have. In the case of the protocols in this manual, there is a bit of art required in the performance of these protocols. But the experience and advice of these authors provides all of the information needed to achieve success in the lab.

Product Identifiers

Publisher
Cold Spring Harbor Laboratory Press,U.S.
ISBN-13
9781936113019
eBay Product ID (ePID)
177059172

Product Key Features

Subject Area
Bioengineering
Author
Richard Behringer
Publication Name
Manipulating the Mouse Embryo: a Laboratory Manual, Fourth Edition
Format
Paperback
Language
English
Subject
Medicine, Biology
Publication Year
2014
Type
Textbook
Number of Pages
814 Pages

Additional Product Features

Title_Author
Richard Behringer
Country/Region of Manufacture
United States

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Manipulating the Mouse Embryo: A Laboratory Manual, Fourth Edition by Richard Behringer (Paperback, 2014)

About this product

Product Information

Product Identifiers

Product Key Features

Additional Product Features

Best Selling in Adult Learning & University

Australian Guidebook for Structural Engineers by Lonnie Pack (Paperback, 2019)

Eduqas GCSE (9-1) Religious Studies Route B: Catholic Christianity and Judaism by Deirdre Cleary, Andrew Barron, Joy White, Patrick Harrison (Paperback, 2017)

The Ethnic Cleansing of Palestine by Ilan Pappe (Paperback, 2007)

Save on Adult Learning & University

The Australian Native Bee Hive Book: Keeping Stingless Sugarbag OATH Tim Heard

Weed Forager's Handbook: A Guide to Edible and Medicinal Weeds in Australia by A

Netter Atlas of Human Anatomy: Classic Regional Approach

The Indoctrinated Brain: How to Successfully Fend Off the Global Attack on Your

George Hrabovsky Leonard Susskind Classical Mechanics (Paperback)

Havard's Nursing Guide to Drugs

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