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Relationship Between Plasmid Copy Number and Antibiotic Resistance by Magapu Solomon Sudhakar, Matta Sri Anusha, Balachander Anitha (Paperback, 2013)

About this product

About this product

Product Information

Plasmids are extrachromosomal DNA which is used in recombinant protein production. The project aims to determine the existence of a relationship between the antibiotic resistance of a clone and the plasmid copy number and the expression of the particular protein. The study was conducted on three clones of Escherichia coli carrying a gene of interest. The three clones are resistant to different concentrations of Ampicillin. Expression studies, Plasmid Isolation and Colony PCR are used to determine this. The results of our experiments showed that there was no difference in expression shown by the three clones. Experiments involving Plasmid Isolation and Colony PCR also do not show a difference between the three clones. It could thus be concluded that the antibiotic resistance may be attributed to other factors.

Product Identifiers

Publisher
Lap Lambert Academic Publishing
ISBN-13
9783659393310
eBay Product ID (ePID)
176393743

Product Key Features

Author
Magapu Solomon Sudhakar, Matta Sri Anusha, Balachander Anitha
Publication Name
Relationship between Plasmid Copy Number and Antibiotic Resistance
Format
Paperback
Language
English
Subject
Chemistry
Publication Year
2013
Type
Textbook
Number of Pages
60 Pages

Dimensions

Item Height
229mm
Item Width
152mm
Item Weight
100g

Additional Product Features

Title_Author
Balachander Anitha, Matta Sri Anusha, Magapu Solomon Sudhakar

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