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Avidin-Biotin Interactions: Methods and Applications by Robert J. McMahon (Engli
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ApproximatelyAU $185.93
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Located in: Fairfield, Ohio, United States
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eBay item number:386679733834
Item specifics
- Condition
- ISBN-13
- 9781617376573
- Book Title
- Avidin-Biotin Interactions
- ISBN
- 9781617376573
- Subject Area
- Science
- Publication Name
- Avidin-Biotin Interactions : Methods and Applications
- Item Length
- 9 in
- Publisher
- Humana Press
- Subject
- Life Sciences / Molecular Biology, Life Sciences / Cell Biology, Life Sciences / Biochemistry, Chemistry / Electrochemistry, Chemistry / Analytic, Chemistry / Organic
- Publication Year
- 2010
- Series
- Methods in Molecular Biology Ser.
- Type
- Textbook
- Format
- Trade Paperback
- Language
- English
- Item Width
- 6 in
- Item Weight
- 13.1 Oz
- Number of Pages
- Xiv, 232 Pages
About this product
Product Information
This book is specifically about the application of the extremely powerful interaction between the protein avidin or its homologues and the vitamin biotin and some of its homologues. With excellent descriptions of laboratory protocols written by expert researchers, this volume is equally perfect for the student or the professional laboratory scientist.
Product Identifiers
Publisher
Humana Press
ISBN-10
1617376574
ISBN-13
9781617376573
eBay Product ID (ePID)
109109300
Product Key Features
Publication Name
Avidin-Biotin Interactions : Methods and Applications
Format
Trade Paperback
Language
English
Subject
Life Sciences / Molecular Biology, Life Sciences / Cell Biology, Life Sciences / Biochemistry, Chemistry / Electrochemistry, Chemistry / Analytic, Chemistry / Organic
Publication Year
2010
Series
Methods in Molecular Biology Ser.
Type
Textbook
Subject Area
Science
Number of Pages
Xiv, 232 Pages
Dimensions
Item Length
9 in
Item Width
6 in
Item Weight
13.1 Oz
Additional Product Features
Intended Audience
Scholarly & Professional
Series Volume Number
418
Number of Volumes
1 Vol.
LeafCats
2228
Lc Classification Number
Qd431-431.7
Reviews
Aus den Rezensionen:"... Das ... editierte Methodenbuch gliedert sich in siebzehn Beiträge, die von auf dem jeweiligen Gebiet ausgewiesenen Autoren verfasst wurden. ... Die angegebenen Protokolle ermöglichen, einem Methodenbuch entsprechend, eine zeitsparende und kostengünstige Präparation ... Positiv ist auch die gute Darstellung der Faktoren, die für Biotinylierungsreaktionen ... kritisch sind. ... Das Buch ist nach Form und Inhalt in jedem Fall geeignet, dem Leser die weitere Vertiefung in die aktuelle Literatur zu ermöglichen und Hinweise für Applikationen zu geben. ... eine umfassende Informationsquelle und Protokolle zur Verfügung ..." (Dr. rer. nat. H. Walzel, in: Laboratoriums-Medizin: journal of laboratory medicine, 2009, Vol.33, Issue 2, S. 129 f.), Aus den Rezensionen:... Das ... editierte Methodenbuch gliedert sich in siebzehn Beiträge, die von auf dem jeweiligen Gebiet ausgewiesenen Autoren verfasst wurden. ... Die angegebenen Protokolle ermöglichen, einem Methodenbuch entsprechend, eine zeitsparende und kostengünstige Präparation ... Positiv ist auch die gute Darstellung der Faktoren, die für Biotinylierungsreaktionen ... kritisch sind. ... Das Buch ist nach Form und Inhalt in jedem Fall geeignet, dem Leser die weitere Vertiefung in die aktuelle Literatur zu ermöglichen und Hinweise für Applikationen zu geben. ... eine umfassende Informationsquelle und Protokolle zur Verfügung ... (Dr. rer. nat. H. Walzel, in: Laboratoriums-Medizin: journal of laboratory medicine, 2009, Vol.33, Issue 2, S. 129 f.), Aus den Rezensionen: S... Das ... editierte Methodenbuch gliedert sich in siebzehn Beitr ge, die von auf dem jeweiligen Gebiet ausgewiesenen Autoren verfasst wurden. ... Die angegebenen Protokolle erm glichen, einem Methodenbuch entsprechend, eine zeitsparende und kosteng nstige Pr paration ... Positiv ist auch die gute Darstellung der Faktoren, die f r Biotinylierungsreaktionen ... kritisch sind. ... Das Buch ist nach Form und Inhalt in jedem Fall geeignet, dem Leser die weitere Vertiefung in die aktuelle Literatur zu erm glichen und Hinweise f r Applikationen zu geben. ... eine umfassende Informationsquelle und Protokolle zur Verf gung ... S (Dr. rer. nat. H. Walzel, in: Laboratoriums-Medizin: journal of laboratory medicine, 2009, Vol.33, Issue 2, S. 129 f.)
Table of Content
Preparation of Avidin Conjugates.- Coupling of Antibodies With Biotin.- Optimization of Detection and Quantification of Proteins on Membranes in Very High and Very Low Abundance Using Avidin and Streptavidin.- Use of the Avidin (Imino)Biotin System as a General Approach to Affinity Precipitation.- Capturing Biotinylated Proteins and Peptides by Avidin Functional Affinity Electrophoresis.- Functionality Screen of Streptavidin Mutants by Non-Denaturing SDS-PAGE Using Biotin-4-Fluorescein.- Application of Biotin-4-Fluorescein in Homogeneous Fluorescence Assays for Avidin, Streptavidin, and Biotin or Biotin Derivatives.- Quantitative Recovery of Biotinylated Proteins from Streptavidin-Based Affinity Chromatography Resins.- High-Yield Production and Purification of Recombinant T7-Tag Mature Streptavidin in Glucose-Stressed E. coli.- Endogenous Biotin in Rat Brain.- Pseudo-Immunolabelling With the Avidin-Biotin-Peroxidase Complex due to the Presence of Endogenous Biotin in the Retina.- Use of Synthetic Peptides for Identifying Biotinylation Sites in Human Histones.- Detection and Quantitation of the Activity of DNA Methyltransferases Using a Biotin/Avidin Microplate Assay.- Anti-Biotin Antibodies Offer Superior Organelle-Specific Labelling of Mitochondria Over Avidin or Streptavidin.- Mammalian Lectin as Tool in Glycochemistry and Histochemistry With Relevance for Diagnostic Procedure.- A Multiplex Approach to Isotyping Antigen-Specific Antibodies Using Biotinylated Antigen/Streptavidin-Phycoerythrin.- Biotin-Protein Bond: Instability and Structural Modification to Provide Stability for In Vivo Applications.
Copyright Date
2008
Dewey Decimal
572.58
Dewey Edition
22
Illustrated
Yes
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