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The P2X7 Receptor: Methods and Protocols by Annette Nicke (English) Hardcover Bo

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eBay item number:395165342956
Last updated on 19 May, 2024 20:25:23 AESTView all revisionsView all revisions

Item specifics

Condition
Brand new: A new, unread, unused book in perfect condition with no missing or damaged pages. See the ...
ISBN-13
9781071623831
Type
NA
Publication Name
NA
ISBN
9781071623831
Book Title
P2x7 Receptor : Methods and Protocols
Book Series
Methods in Molecular Biology Ser.
Publisher
Springer
Item Length
10 in
Publication Year
2022
Format
Hardcover
Language
English
Illustrator
Yes
Author
Annette Nicke
Genre
Science, Medical
Topic
Immunology, Life Sciences / Biochemistry, Pharmacology, Life Sciences / Anatomy & Physiology (See Also Life Sciences / Human Anatomy & Physiology)
Item Weight
33.9 Oz
Item Width
7 in
Number of Pages
Xiv, 394 Pages

About this product

Product Information

This detailed volume covers diverse aspects of P2X7 receptor analysis, ranging from its molecular structure to related pharmacological and immunological tools, via its analysis in heterologous expression systems as well as assays using primary cells and whole animal models. After three introductory chapters that focus on its structure, ligands, and physiological functions, the book details the generation of antibody and nanobody tools for P2X7 receptors, provides protocols for the analysis of expressed P2X7 receptors with a focus on their electrophysiological analysis, as well as protocols for the investigation of P2X7 down-stream signaling in immune cells by flow cytometry. Mouse models and procedures suited to investigate P2X7-mediated effects in other primary cells and in vivo are also explained . Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, The P2X7 Receptor: Methods and Protocols is a valuable reference not only for the growing community fascinated by this unusual ion channel but also for a broad readership interested in ion channels or purinergic receptors.

Product Identifiers

Publisher
Springer
ISBN-10
1071623834
ISBN-13
9781071623831
eBay Product ID (ePID)
2328293994

Product Key Features

Book Title
P2x7 Receptor : Methods and Protocols
Number of Pages
Xiv, 394 Pages
Language
English
Topic
Immunology, Life Sciences / Biochemistry, Pharmacology, Life Sciences / Anatomy & Physiology (See Also Life Sciences / Human Anatomy & Physiology)
Publication Year
2022
Illustrator
Yes
Genre
Science, Medical
Author
Annette Nicke
Book Series
Methods in Molecular Biology Ser.
Format
Hardcover

Dimensions

Item Weight
33.9 Oz
Item Length
10 in
Item Width
7 in

Additional Product Features

Dewey Edition
23
Series Volume Number
2510
Number of Volumes
1 Vol.
Dewey Decimal
547.596
Lc Classification Number
Qd415-436
Table of Content
1. How Structural Biology Has Directly Impacted Our Understanding of P2X Receptor Function and Gating Steven E. Mansoor 2. Agonists, Antagonists, and Modulators of P2X7 Receptors Christa E. Müller and Vigneshwaran Namasivayam 3. Modulation of Cell Energy Metabolism by the P2X7 Receptor Francesco Di Virgilio, Simonetta Falzoni, Alba Clara Sarti, Paola Chiozzi, Valentina Vultaggio-Poma, and Anna Lisa Giuliani 4. Fully Flexible Ligand Docking for the P2X7 Receptor Using ROSIE Sudad Dayl and Ralf Schmid 5. Preparation of the Murine Anti-Human P2X7 Receptor Monoclonal Antibody (Clone L4) Amal Elhage, Ross J. Turner, Peter Cuthbertson, Debbie Watson, and Ronald Sluyter 6. Development of Antibody and Nanobody Tools for P2X7 Tobias Stähler, Welbeck Danquah, Melanie Demeules, Henri Gondé, Romain Hardet, Friedrich Haag, Sahil Adriouch, Friedrich Koch-Nolte, and Stephan Menzel 7. Enhanced Transduction of P2X7-Expressing Cells with Recombinant rAAV Vectors Anna Marei Mann, Waldemar Schäfer, Sahil Adriouch, Kathleen Börner, Dirk Grimm, Ingke Braren, and Friedrich Koch-Nolte 8. Immunofluorescence Staining of P2X7 Receptors in Whole-Mount Myenteric Plexus Preparations Tina Jooss, Birgit Kuch, Jiong Zhang, Michael Schemann, and Annette Nicke 9. Established Protocols for cRNA Expression and Voltage-Clamp Characterization of the P2X7 Receptor in Xenopus laevis Oocytes Günther Schmalzing and Fritz Markwardt 10. A Simplified Protocol to Incorporate the Fluorescent Unnatural Amino Acid ANAP into Xenopus laevis Oocyte-Expressed P2X7 Receptors Anna Durner and Annette Nicke 11. Using Whole-Cell Electrophysiology and Patch Clamp Photometry to Characterize P2X7 Receptor Currents Xin Liang, Laura Janks, and Terrance M. Egan 12. Using Symmetrical Organic Cation Solutions to Study P2X7 Ion Permeation Kate Dunning, Laurie Peverini, and Thomas Grutter 13. Methods for Studying Cholesterol-Dependent Regulation of P2X7 Receptors Ruth D. Murrell-Lagnado and Toshimitsu Kawate 14. Visualizing P2X7-Dependent Inflammasome Formation in Human Monocytes by Fluorescence Microscopy and Flow Cytometry Samantha Wittke, Leif Ancker, Sana Javed, and Friedrich Haag 15. Characterization of P2X7 Receptors in Human Blood Cells Laura Hurtado-Navarro, Alberto Baroja-Mazo, and Pablo Pelegrín 16. Real-Time Flow Cytometry as a Tool to Monitor Cellular Consequences of P2X7 Activation in Multiple Cell Populations Mixed in a Single FACS Tube Alexander W. Veltkamp, Tim Magnus, and Björn Rissiek 17. Administration of P2X7 Receptor Blockers in Oncological Experimental Models Elena De Marchi, Anna Pegoraro, and Elena Adinolfi 18. Humanized Mouse Model to Study the P2X7 Receptor in Graft-Versus-Host Disease Debbie Watson, Sam R. Adhikary, Peter Cuthbertson, Nicholas J. Geraghty, Katrina M. Bird, Amal Elhage, Chloe Sligar, and Ronald Sluyter 19. Analysis of P2X7-Induced Neuronal Branching Paula Mut-Arbona and Beáta Sperlágh 20. Studying the Role of P2X7 Receptor in Axonal Growth Using In Utero Electroporation Technique Álvaro Sebastián-Serrano, Carolina Bianchi, Caterina Di Lauro, Lucia Soria-Tobar, Beatriz Álvarez-Castelao, and Miguel Díaz-Hernández 21. Analyzing the Role of the P2X7 Receptor in Epilepsy Mariana Alves, Laura de Diego-Garcia, and Tobias Engel
Copyright Date
2022

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