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Two Hybrid Technologies: Methods and Protocols by Erich E. Wanker, Bernhard Suter (Hardcover, 2012)

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Product Information

Protein-protein interactions (PPIs) are strongly predictive of functional relationships among proteins in virtually all processes that take place in the living cell. Therefore, the comprehensive exploration of interactome networks is one of the major goals in systems biology. The aim of Two Hybrid Technologies: Methods and Protocols is to provide a compendium of state-of-the art protocols for the investigation of binary PPIs with the classical yeast two-hybrid (Y2H) approach, Y2H variants and other in vivo methods for PPI mapping. Divided into two convenient sections, the first gives a survey of protocols that are currently employed for Y2H high-throughput screens by different expert labs in the field. Rather than detailing the principles of screening, which have been described previously, the focus is on different implementations of Y2H interactome mapping. The second section of the book considers innovative PPI detection methods that have the potential to emerge as alternative high-throughput methodologies. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Two Hybrid Technologies: Methods and Protocols supplies researchers with a comprehensive toolbox for the identification of biologically relevant protein interactions.

Product Identifiers

Publisher
Humana Press Inc.
ISBN-13
9781617794544
eBay Product ID (ePID)
111531654

Product Key Features

Author
Erich E. Wanker, Bernhard Suter
Publication Name
Two Hybrid Technologies: Methods and Protocols
Format
Hardcover
Language
English
Subject
Chemistry
Publication Year
2012
Type
Textbook
Number of Pages
329 Pages

Dimensions

Item Height
254mm
Item Width
178mm
Volume
812
Item Weight
845g

Additional Product Features

Series Title
Methods in Molecular Biology
Country/Region of Manufacture
United States
Editor
Bernhard Suter, Erich E. Wanker

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Two Hybrid Technologies: Methods and Protocols by Erich E. Wanker, Bernhard Suter (Hardcover, 2012)

About this product

Product Information

Product Identifiers

Product Key Features

Dimensions

Additional Product Features

Best Selling in Adult Learning & University

Permaculture: A Designer's Manual by Bill Mollison (Hardcover, 1988)

Australian Native Bee Book: Keeping Stingless Bee Hives for Pets, Pollination and Sugarbag Honey by Tim Heard (Paperback, 2015)

The Weed Forager's Handbook: A Guide to Edible and Medicinal Weeds in Australia by Adam Grubb, Annie Raser-Rowland (2012, Paperback)

In-Basket Case Studies by Guy Adamson (Paperback, 1986)

Empire of the Summer Moon: Quanah Parker and the Rise and Fall of the Comanches, the Most Powerful Indian Tribe in American History by S.C. Gwynne (Paperback, 2011)

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A guide to the Project Management Body of Knowledge (PMBOK guide) and the Standard for project management by Project Management Institute (Paperback, 2021)

Save on Adult Learning & University

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