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Directed Evolution Library Creation: Methods and Protocols by George Georgiou, Frances H. Arnold (Hardcover, 2003)

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A comprehensive compendium of cutting-edge protocols for the generation of molecular diversity. Described in step-by-step detail to ensure experimental success, these protocols include readily reproducible methods for random mutagenesis of entire genes or segments of genes, for homologous and nonhomologus recombination, and for constructing in vivo libraries in bacteria and yeast. In addition to the various protocols for creating libraries, this volume also describes ways to analyze libraries, particularly those made by recombination. An accompanying volume, Directed Enzyme Evolution: Screening and Selection Methods (ISBN: 1-58829-286-X), is devoted entirely to selection and screening methods that can be applied to the directed evolution of enzymes. Copy for Both Volumes Directed Evolution Library Creation: Methods and Protocols and Directed Enzyme Evolution: Screening and Selection Methods constitute an extraordinary collection of all the key methods used today for directed evolution research. Described in step-by-step detail to ensure robust experimental results, these methods will enable both newcomers and more experienced investigators to design and implement directed evolution strategies for the engineering of novel proteins. The first volume describes methods for the creation of mutated DNA molecules, or DNA libraries, encoding variants of desired proteins. The second volume describes methods for screening DNA libraries to isolate mutant proteins that exhibit a specified function.

Product Identifiers

Publisher
Humana Press Inc.
ISBN-13
9781588292858
eBay Product ID (ePID)
96711664

Product Key Features

Author
George Georgiou, Frances H. Arnold
Publication Name
Directed Evolution Library Creation: Methods and Protocols
Format
Hardcover
Language
English
Subject
Biology
Publication Year
2003
Type
Textbook
Number of Pages
226 Pages

Dimensions

Item Height
229mm
Item Width
152mm
Volume
231
Item Weight
542g

Additional Product Features

Series Title
Methods in Molecular Biology
Country/Region of Manufacture
United States
Editor
George Georgiou, Frances H. Arnold

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Directed Evolution Library Creation: Methods and Protocols by George Georgiou, Frances H. Arnold (Hardcover, 2003)

About this product

Product Information

Product Identifiers

Product Key Features

Dimensions

Additional Product Features

Best Selling in Adult Learning & University

Australian Guidebook for Structural Engineers by Lonnie Pack (Paperback, 2019)

Eduqas GCSE (9-1) Religious Studies Route B: Catholic Christianity and Judaism by Deirdre Cleary, Andrew Barron, Joy White, Patrick Harrison (Paperback, 2017)

Time Management by Peter Quarry (Paperback, 1986)

The Ethnic Cleansing of Palestine by Ilan Pappe (Paperback, 2007)

Save on Adult Learning & University

The Australian Native Bee Hive Book: Keeping Stingless Sugarbag OATH Tim Heard

Weed Forager's Handbook: A Guide to Edible and Medicinal Weeds in Australia by A

Netter Atlas of Human Anatomy: Classic Regional Approach

Classical Mechanics: The Theoretical Minimum by George Hrabovsky

Havard's Nursing Guide to Drugs

T. Jared Bunch Matthew LaPlante John D. Day The AFib Cure (Paperback)

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