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Galectins: Methods and Protocols by Richard D. Cummings, Sean R. Stowell (Hardcover, 2014)

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Evaluation of Galectin Binding by Frontal Affinity Chromatography (FAC). - Evaluation of Galectin Binding by Surface Plasmon Resonance. - Examining Galectin Binding Specificity Using Glycan Microarrays.

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Galectins: Methods and Protocols is the first book solely dedicated to methodological approaches designed to study galectin function. The galectin family represents one of the most pleiotropic families, with individual members having been implicated in various aspects of nearly every biological process described, from RNA splicing to complex regulatory circuits that orchestrate adaptive immunity. Given the diverse roles of galectins in a variety of biological systems, studying these glycan binding proteins often requires the assimilation of diverse technical skills to fully appreciate their biological function. Their nearly ubiquitous expression and ability to bind highly modifiable carbohydrate ligands, in addition to a variety of other regulatory proteins, allows these glycan binding proteins (GBPs) to possess the capacity to regulate a wide variety of biological processes. Individual chapters are dedicated to examining salient features of galectin functions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tes on troubleshooting and avoiding kwn pitfalls. Authoritative and easily accessible, Galectins: Methods and Protocols seeks to serve both professionals and vices with a useful framework when examining galectin function for many years to come.