Manipulating the Mouse Embryo: A Laboratory Manual, Fourth Edition by Andras Nagy, Richard Behringer, Kristina Nagy, Marina Gertsenstein (Hardback, 2013)
This 4th edition of the Mouse Manual -Manipulating the Mouse Embryo-appears 28 years after the first edition and once again is the definitive reference source on mouse development, transgenesis techniques, and molecular biology. Authors Richard Behringer, Marina Gertsenstein, Kristina Nagy, and Andras Nagy-pre-emininent leaders in their fields-have reorganized and updated this edition to include new information and protocols on: * assisted reproduction techniques for sperm and embryo cryopreservation * generation of induced pluripotent stem cells * isolation, generation, and transplantation of spermatogonial stem cell lines * in utero electroporation of gene constructs into post- implantation embryos * vibratome sectioning of live and fixed tissues for imaging thick tissue sections * whole-mount fluorescent staining methods for three-dimensional visualization. Techniques regarding recombinant DNA techlogy and mouse embryonic development from the previous editions have been updated and recast, as has the wealth of information on mouse laboratory strains, mouse housing and breeding, surgical procedures, assisted reproduction, handling of embryos, and micromanipulation setups. The first edition of Manipulating the Mouse Embryo appeared in 1986 as an outgrowth of Cold Spring Harbor Laboratory courses on the molecular embryology of the mouse held in the early 1980s, and authors of the first two editions included Brigid Hogan, Rosa Beddington, Frank Costantini, and Liz Lacy. Mouse embryo manipulation techniques have developed exponentially since the first edition, but then, as w, Manipulating the Mouse Embryo remains the essential practical and theoretical guide for anyone working with mice-students, lab technicians, and investigators.
Product Identifiers
Publisher
Cold Spring Harbor Laboratory Press
ISBN-10
1936113007
ISBN-13
9781936113002
eBay Product ID (ePID)
190170293
Product Key Features
Author
Richard Behringer, Andras Nagy, Marina Gertsenstein, Kristina Nagy