Protein engineering is the rational modification or redesign of proteins using genetic engineering. Thus, it is now possible to modify enzyme specificities, remodel antibodies, and redesign many multi-domain proteins for therapeutic purposes. While the procedures for the introduction of mutations have become routine, predicting and understanding the effects of these mutations can be complicated. This volume provides a comprehensive guide to the methods used at every stage of the engineering process, from the choice of mutation strategy, through protein stability studies, to critical evaluations of mammalian, yeast, and bacterial host expression systems. Protein Engineering: A Practical Approach is the first practical guide to this fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry. It combines a thorough theoretical foundation with detailed protocols and will be invaluable to all research workers in the area, from graduate students to senior investigators.
Product Identifiers
Publisher
Oxford University Press
ISBN-13
9780199631384
eBay Product ID (ePID)
95003530
Product Key Features
Subject Area
Bioengineering
Author
Anthony R. Rees, Ronald Wetzel, Michael J. E. Sternberg
Publication Name
Protein Engineering: a Practical Approach
Format
Paperback
Language
English
Subject
Chemistry
Publication Year
1992
Type
Textbook
Number of Pages
424 Pages
Dimensions
Item Height
234mm
Item Width
156mm
Volume
108
Item Weight
646g
Additional Product Features
Series Title
Practical Approach Series
Editor
Anthony R. Rees, Michael J. E. Sternberg, Ronald Wetzel