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Stem Cell Bioprocessing: For Cellular Therapy, Diagnostics and Drug Development by Maria Margarida Diogo, Tiago Fernandes, Joaquim M. S. Cabral (Hardback, 2013)
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About this product
Description
- DescriptionStem cell bioprocessing describes the main large-scale bioprocessing strategies for both stem cell culture and purification, envisaging the application of these cells for regenerative medicine and drug screening. Bioreactor configurations are described, including their applications for stem cell expansion, and stem cell separation techniques such as isolation and purification are discussed. Basic definitions are provided concerning the different types of stem cells, from adult stem cells to the more recent induced pluripotent stem cells. The main characteristics of these different stem cell types are described, alongside the molecular mechanisms underlying their self-renewal and differentiation. The book also focuses on methodologies currently used for in vitro stem cell culture under static conditions, including the challenge of xe-free culture conditions, as well as culture parameters that influence stem cell culture. Approaches for both stem cell culture and separation in micro-scale conditions are presented, including the use of cellular microarrays for high-throughput screening of the effect of both soluble and extracellular matrix molecules. A further section is dedicated to application of stem cells for regenerative medicine.
- Author BiographyDr Tiago G. Fernandes is currently a Post-doctoral research fellow at the Institute for Biotechnology and Bioengineering, Lisbon, Portugal. His research interests are focused on the development of artificial cellular niches for studying the mechanisms that affect human stem cell pluripotency, and the cellular and molecular events that regulate stem cell functions. During his doctoral studies he also worked at the Rensselaer Polytechnic Institute, NY, USA, in the development of microscale platforms for high-throughput studies of stem cell fate. Dr Maria Margarida Diogo received her PhD degree in Biotechnology from Instituto Superior Tecnico (IST) from January 2000 to April 2004. Her thesis was dedicated to the development of Bioprocesses for Production and Purification of Plasmid DNA for Gene Therapy and DNA vaccines. As a result of this work, a United States Patent was published in 2007. From April 2004 to March 2008 she received a post-doctoral fellowship to work in the field of Stem Cell Bioengineering and she is presently a Research Scientist at the Stem Cell Bioengineering Laboratory at Instituto Superior Tecnico (IST). Dr Joaquim M. S. Cabral is Professor of Biological Engineering, in the Department of Bioengineering at Instituto Superior Tecnico (IST), Technical University of Lisbon, Portugal. He was the founder and current Head of Department of Bioengineering, and the Director of the Institute for Biotechnology and Bioengineering, a Research Laboratory Associated to the Ministry of Science, Technology and Higher Education in Portugal. His research aims at contributing to biochemical engineering science through novel developments in Bioprocess Engineering and Stem Cell Bioengineering.
Key Features
- Author(s)Joaquim M. S. Cabral,Maria Margarida Diogo,Tiago Fernandes
- PublisherElsevier Science & Technology
- Date of Publication07/10/2013
- LanguageEnglish
- FormatHardback
- ISBN-101907568883
- ISBN-139781907568886
- SubjectMedical Nursing & Ancillary Services
- ISSN2050-0289
- Series TitleWoodhead Publishing Series in Biomedicine
- Series Part/Volume NumberNo. 40
Publication Data
- Place of PublicationCambridge
- Country of PublicationUnited Kingdom
- ImprintWoodhead Publishing Ltd
- Content Noteblack & white illustrations
Dimensions
- Weight510 g
- Width156 mm
- Height234 mm
- Spine14 mm
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